Effects of e-cigarette aerosol on oral epithelial cell metabolism

Although electronic cigarette (e-cig) has been suggested as a substitute for conventional smoking or as a tool for smoking cessation, limited knowledge is available about its safety and potential risk to oral health. We have recently demonstrated that e-cig induces oxidative stress and unfolded protein responses in human oral epithelial cells, leading to significant cytotoxicity. In this project, we aim to conduct a thorough investigation of the effects of e-cig on the cellular metabolism of human oral cavity using advanced metabolomics technology. Successful conclusion of our proposed studies will reveal important molecular mechanisms underlying e-cig toxicology and provide critical knowledge about the potential risk of e-cig in oral diseases and dental health. The expected findings of our project will be valuable for e-cig regulatory decision making and provide guidance for safety assessment of e-cig and improved e-cig product design.